[Histonet] Re: Which one first - antigen retrieval or peroxidase block


I'm with Rene on this one.  Block after retrieval.
For those of you who do the block before retrieval, how do you  control for 
any activity that might be exposed during the retrieval  step?  Couldn't it 
lead to false positives in your slides? 
Albert C.  Grobe, PhD
Tissue Engineering Lab
International Heart Institute of Montana  Foundation

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