Greetings all,

 

I'm wondering whether there are any recommendations for how to
effectively cryosection dorsal mouse skin for immunohistochemistry. I'm
having difficulty with much of the mouse skin slipping off the slide
during the fixation and washing steps, which seems to result in loss of
the epidermis in particular, that part of the tissue that I happen to be
interested in. I apply the dorsal mouse skin cryosections onto
SuperFrost Plus (Menzel-Glaser) slides and air dry them for 2 hours. I
use acetone for fixation, 10 minutes, and use PBS for washing. Any
suggestions?



This email (including any attachments or links) may contain 
confidential and/or legally privileged information and is 
intended only to be read or used by the addressee.  If you 
are not the intended addressee, any use, distribution, 
disclosure or copying of this email is strictly 
prohibited.  
Confidentiality and legal privilege attached to this email 
(including any attachments) are not waived or lost by 
reason of its mistaken delivery to you.
If you have received this email in error, please delete it 
and notify us immediately by telephone or email.  Peter 
MacCallum Cancer Centre provides no guarantee that this 
transmission is free of virus or that it has not been 
intercepted or altered and will not be liable for any delay 
in its receipt.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet