Methylene blue does not require anything special, this stain never fails. Just hydrate your section, stain with 1% aq. methBlue for 5 minutes, wash and dehydrate QUICKLYLY cover and that is all. wrote:


From: Perry, Margaret 
Subject: [Histonet] methylene blue
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 9, 2008, 4:22 PM

Help!     We have been asked to do a methylene blue stain for
Myxosporidia and don't have a procedure.  Of course the pathologist
wants it quickly.  I can't get into the archives.  It must be down right
now.  Can someone help us?

 

 

Margaret Perry HT (ASCP)

IHC Lab Manager Veterinary Science

Animal Disease Research and Diagnostic Lab

South Dakota State University

Box 2175 North Campus Drive

Brookings SD 57007

 

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