Are your PAP smear slides fixed in alcohol?  If so, you probably don't need antigen retrieval.

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "hao zhang"  09/13/08 11:11 PM >>>
Hi, everyone

I want to do IHC from a 5 year old pap smear slides for pan-keratin stain.

I tried many methods of antigen retrieval protocols (10 mM citric buffer, ph
6; 1 mM EDTA, Ph8; 10 mM Tris buffer, Ph 10; ) and heat at 95C for 40
minutes. I also used Triton x 100 (0.2%) pretreatment 10 minutes and then
SDS (0.5% or 0.25%) treatment for 10 minutes. But all method did not work.
Any replies will be very appreciated.


-- 
Hao ZHANG, Ph.D

Scientist
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