Re: [Histonet] antigen retrieval for 5 year pap smear slides

From:Rene J Buesa

Usually PAP smears are NOT fixed with NBF so they should NOT be treated with HIER before doing IHC. If you have some spare smear do IHC directly (after destaining).
René J.

--- On Sat, 9/13/08, hao zhang  wrote:

From: hao zhang 
Subject: [Histonet] antigen retrieval for 5 year pap smear slides
Date: Saturday, September 13, 2008, 11:11 PM

Hi, everyone

I want to do IHC from a 5 year old pap smear slides for pan-keratin stain.

I tried many methods of antigen retrieval protocols (10 mM citric buffer, ph
6; 1 mM EDTA, Ph8; 10 mM Tris buffer, Ph 10; ) and heat at 95C for 40
minutes. I also used Triton x 100 (0.2%) pretreatment 10 minutes and then
SDS (0.5% or 0.25%) treatment for 10 minutes. But all method did not work.
Any replies will be very appreciated.


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