Re: [Histonet] What to do when there is no liquid nitrogen :p

From:"Bob Nienhuis"



At our last safety class they showed pictures of a couple of blown-up
hoods and cabinets caused by exploding isopentane.
Especially dangerous when there is only a small amount in the bottle
so that vapors can form. Flash point in near room temperature.

Bob


On 9/3/08, Heath, Nancy L.  wrote:
>
> Hi Everyone,
>
> Does anyone have alternate methods for snap freezing muscle when there
> is no liquid nitrogen?? I've been told to make a slurry with isopentane
> & dry ice or to put isopentane in a plastic cup (?) and put into the
> minus 70* freezer till it gets cold. Also how volatile / dangerous is
> isopentane to work with??? Other than a flammable cabinet are there any
> other things I need to know about isopentane???
>
> Thanks,
> Nancy
>
>
>
>
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