Re: [Histonet] VEGFR1 and 2

From:"Nancy Lemke"

Hi Sarah,
I can heartily recommend Santa Cruz sc-6251.  FFPE: retrieve in citrate buffer, pH 6.0- boil for 10 minutes, cool for 20.  Block, then incubate in sc-6251 at 1:1500 for 30 minutes at room temp.  I use Biocare 4+ detection as given on data sheet and Biocare Betazoid DAB for 4 minutes and get beautiful, clean results.  I use this in rat sections.

Nancy Lemke
Research Coordinator
Hermelin Brain Tumor Center
Henry Ford Hospital
-----Original message-----
From: "Hale, Sarah"
Date: Mon, 08 Sep 2008 13:37:43 -0400
Subject: [Histonet] VEGFR1 and 2

> Hi!
> Does anyone have any experience with IHC for VEGFR1 and 2 in rat
> arteries?  Our lab has had trouble getting good staining especially with
> paraffin sections.
> Thanks!
> -Sarah
> __________________________________________________
> Sarah Hale, Ph.D.
> Postdoctoral Associate
> Dept. of OB/Gyn and Reproductive Sciences
> C215 Given Bldg.
> University of Vermont
> Burlington, VT  05405
> ph: 802.656.1207
> email:
> _______________________________________________
> Histonet mailing list

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