Are you putting the tissue into media like RPMI or DMEM to keep it viable
until you use it?
P.S. Joyce, I loved the lipstick comment hehe
On Thu, Sep 11, 2008 at 11:42 AM, Alistair Lindsay <
> Dear All,
> Does anyone have any experience with staining pig endothelium, specifically
> keeping rings of blood vessels alive?
> I am collecting fresh tissue from the slaughterhouse and incubating it with
> tumour necrosis factor to induce VCAM-1 expression. However, I suspect
> the tissue quickly loses viability and this is why I canšt visualise any
> VCAM with IHC.
> Does anyone have any tips for keeping the rings of tissue
> going/fixing/antigen retrieval?
> Many thanks
> Alistair Lindsay MBChB MRCP
> Tel.: 07989404808
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