Are you putting the tissue into media like RPMI or DMEM to keep it viable
until you use it?

P.S.   Joyce, I loved the lipstick comment hehe

On Thu, Sep 11, 2008 at 11:42 AM, Alistair Lindsay <
Alistair.Lindsay@btinternet.com> wrote:

> Dear All,
>
> Does anyone have any experience with staining pig endothelium, specifically
> keeping rings of blood vessels alive?
>
> I am collecting fresh tissue from the slaughterhouse and incubating it with
> tumour necrosis factor to induce VCAM-1 expression.  However, I suspect
> that
> the tissue quickly loses viability and this is why I canšt visualise any
> VCAM with IHC.
>
> Does anyone have any tips for keeping the rings of tissue
> going/fixing/antigen retrieval?
>
> Many thanks
>
> Alistair Lindsay MBChB MRCP
>
> Tel.: 07989404808
>
> Alistair.Lindsay@btinternet.com
> Alistair.Lindsay@cardiov.ox.ac.uk
>
>
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