Merced:
Processing and embedding tissues manually is still done occasionally here in the US and specially abroad. As a matter of fact 2% us US labs and 14% of foreign labs routinely manually process their tissues.
 
It implies running the tissues through all the dehydration, clearing and infiltration steps to finally prepare the blocks, also manually, using melted paraffin and dispensed in paper molds, or using Leuckhart rectangles. 
The description would be very long for an e-mail, so my advise is to get a histotechnique book, like Bolles-Lee's "Microtomist Vade-Mecum", or Peter Gray's "The Microtomist's formulary and guide", both are for sale at Amazon.com/books or you may find a copy at the University of Buffalo.
René J.

--- On Tue, 9/9/08, Merced Leiker  wrote:

From: Merced Leiker 
Subject: [Histonet] Manual Paraffin Embedding
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 9, 2008, 4:54 PM

Does anyone process and embed tissues manually instead of using automated=20
and expensive equipment?  Can you tell me how you do it?  Thanks.

Merced


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