You can always "open" the antigenic sites with enzymatic digestions. The antigen retrieval is, by definition, using heat, and sometimes additional pressure to have an even higher heat level.
René J.

--- On Fri, 9/5/08, njoydobro@aol.com  wrote:

From: njoydobro@aol.com 
Subject: [Histonet] Antigen Retreival
To: Histonet@lists.utsouthwestern.edu, ihcrg@googlegroups.com
Date: Friday, September 5, 2008, 11:06 AM

Does anyone out there have any methods of antigen retreival not using heat?

Thanks,

Gene
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