Re: [Histonet] AZF fixed bone marrows and IHC's

From:"Mark Tarango"

I had the same problem but our cores were fixed in 10% NBF.  I tried
everything I could think of to get it to work as far as retrieval, so I
ended up having to do a real TUNEL assay.  We had to assume the antibody
wasn't specific enough.  Same antibody and staining platform as you.


On Fri, Sep 12, 2008 at 12:24 PM, Christine Jacobs  wrote:

> Is anyone having problems with IHC staining after a combination os AZF
> fixation and decal? There was a recent string on the Histonet regarding
> B-Plus but I can't remember exactly what was discussed. We are experiencing
> problems with TdT staining. We get nothing on the core biopsies and only
> edge artifact staining on most clots. The FFPE thymus tissue looks GREAT. We
> are using Cell Marque's predilute antibody on the Ventana BenchMark XT.
> Any and all comments will be appreciated.
> Chris Jacobs, HT(ASCP), QIHC
> Jason Wheaton, HTL (ASCP)
> NorDx Labs
> Scarborough, ME
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