RE: [Histonet] necrosis

From:"Tony Henwood"

A good H&E is easiest.

I have used poly T mRNA in situ hybridisation to demonstrate areas of
necrosis quite well (absence of signal)


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145 

-----Original Message-----
[] On Behalf Of
Christian Franci
Sent: Tuesday, 16 September 2008 7:11 AM
Subject: [Histonet] necrosis

Hello Histonetters!
I was just wondering what is the best way to look for necrosis on FFPE  
tissue sections.
Is H&E the best way or are there other ways to visualize it that are 
more striking?  I need to take lots of photos for presentations and I'm 
searching for a stain/ IHC that is easy to see.

Thanks a bunch,

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