RE: [Histonet] ?bacterial/fungal contamination on slides

From:"Laurie Colbert"

We see this every once in a while, and have traced it to the water
stations on our stainer.  They are supposed to be cleaned with bleach
once a week, but that wasn't happening.  Another time, we had
contamination on the spigot of our 5 gallon deionized water bottle.  We
are supposed to clean that with bleach quarterly.

Laurie Colbert

-----Original Message-----
[] On Behalf Of Beverly
Sent: Thursday, September 18, 2008 4:09 AM
Subject: [Histonet] ?bacterial/fungal contamination on slides

We are experiencing contamination of  GMS and PAS-F stained slides with
a filamentous microorganism, bacterial or possibly fungal. Our flotation
baths and stainers are cleaned daily with Sparkleen and water. We use
distilled water to fill the baths. We use the Ventana system for the
stains, which is decontaminated regularly. Anyone one else experience
this? Any suggestions to the cause of this. 


Beverly Rowe, RT.

Pathology Quality Management Coordinator

Pathology Dept.Rm.2423

St.Clare's Mercy Hospital

Eastern Health

St. John's, NL




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