Yes, you can use isopentane-dry ice slurry, or dry ice and ethanol.
Yes, Isopentane is very volatile and very, very flammable.
No, for heaven's sake don't put it in your -80 freezer. The dry ice will chill it down just fine.
University of Washington
Department of Pathology
> Date: Wed, 3 Sep 2008 11:59:59 -0400
> From: NHeath@Lifespan.org
> To: firstname.lastname@example.org
> Subject: [Histonet] What to do when there is no liquid nitrogen :p
> Hi Everyone,
> Does anyone have alternate methods for snap freezing muscle when there
> is no liquid nitrogen?? I've been told to make a slurry with isopentane
> & dry ice or to put isopentane in a plastic cup (?) and put into the
> minus 70* freezer till it gets cold. Also how volatile / dangerous is
> isopentane to work with??? Other than a flammable cabinet are there any
> other things I need to know about isopentane???
> Histonet mailing list
Stay up to date on your PC, the Web, and your mobile phone with Windows Live.
Histonet mailing list