Gene
Try 1%SDS/PBS for 5 min. Rinse well. Works for some antibodies that
recognize denatured epitopes.
Jon


Jonathan Wilson
Ecofisiologia-CIIMAR
Rua dos Bragas 289
4050-123 Porto Portugal
tel +351 22 340 1809
fax + 351 22 339 0608


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
njoydobro@aol.com
Sent: Friday, September 05, 2008 4:07 PM
To: Histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com
Subject: [Histonet] Antigen Retreival

Does anyone out there have any methods of antigen retreival not using heat?

Thanks,

Gene
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