Dorothy,

We've done several comparisons like this for amyloid staining. In our
experience, 8um works fine. We did see stronger staining (birefringence) in
some thicker sections with Congo Red but I don't think there would be a
significant improvement in staining (sensitivity?) from 8um to 10um. I
wouldn't cut sections any thinner than 8um but you can still get amyloid
staining at 4um. It would be relatively easy to take your controls and cut
them different thicknesses just to prove the point. Good luck!


Mark A Burton HTL ASCP
Lab Manager & Sr. Histotechnologist
Molecular Aging & Development Lab
Boston University Medical Campus

 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb,
Dorothy L
Sent: Tuesday, September 16, 2008 11:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Amyloid

We have been cutting our tissue for amyloid staining @ 8 microns.  One
of my pathologists heard that 10 microns is now standard  and to use a
negative and weakly positive control.  Does anyone have any new
information in this area?  Thanks ahead of time!

Dorothy Webb, HT (ASCP)
Histology Technical Supervisor 
Regions Hospital, Pathology Department 
640 Jackson Street, Saint Paul, MN 55101-2595 
Phone: 651-254-2962
Fax: 651-254-2741 
Regions Hospital is part of the HealthPartners family of care
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