Ozone may indeed be of some concern. Some research notes:
"High levels of atmospheric ozone in Florence air correlated with DNA damage, and to the prevalence of inflammatory pathologies of the upper respiratory tract, although the ozone concentrations were below the Italian recommended attention level. Furthermore, higher levels of DNA damage were correlated with a dysfunction in the ability to maintain a normal epithelial cell structure. These data suggest an association between ozone air levels and damage in the upper respiratory tract. It remains unclear whether ozone itself or other associated pollutants are responsible for the observed alterations." (Environ. Mol. Mutagen. 42:127-135, 2003)
"Beagle dogs exposed for 8 hours a day to 3 ppm O3 for 18 months showed cytologic changes that indicate metabolic alterations. The endoplasmic reticulum of the type 2 alveolar epithelial cells in the proximal alveoli were frequently dilated and contained a moderately electron-dense substance having a periodicity of approximately 754 A. This condition was accompanied by a substantial reduction in the lamellar membranes in the characteristic lamellar bodies of the type 2 cells, suggesting a sequestering of protein in the endoplasmic reticulum. In addition, endothelial cells contained paracrystalline arrays of cytoplasmic membranes not observed in control animals" (Am J Pathol. 1973 December; 73(3): 711-726)
"The interaction of ozone with some molecules results in an increased production of free radicals. The objective of this study was to identify whether acute ozone exposure to 1-1.5 ppm for 4 h, produced cytological and ultrastructural modifications in the olfactory bulb cells. The results showed that in rats exposed to ozone there was a significant loss of dendritic spines on primary and secondary dendrites of granule cells, whereas the control rats did not present such changes. Besides these exposed cells showed vacuolation of neuronal cytoplasm, swelling of Golgi apparatus and mitochondrion, dilation cisterns of the rough endoplasmic reticulum. These findings suggest that oxidative stress produced by ozone induces alterations in the granule layer of the olfactory bulb, which may be related to functional modifications" (Neuroscience Letters Volume 274, Issue 1, 15 October 1999, Pages 1-4)
"Inhalation of the pulmonary irritant ozone is associated with an accumulation of macrophages in the lung. These cells, along with type II epithelial cells, are activated to release increased quantities of hydrogen peroxide and nitric oxide, two reactive mediators that have been implicated in tissue injury" (Am J Respir Cell Mol Biol. 1996 Jun ;14 (6):516-25).
"ozone-induced high-protein alveolar edema is pathogenetically linked to pulmonary hyperemia, deficiency of surfactant tubular myelin, and associated lung dysfunctions" (Toxicol Appl Pharmacol Vol. 117 Issue 1 Pg. 37-45 Nov 1992).
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, 9 September 2008 2:47 AM
To: histonet@lists.utsouthwestern.edu; Margaryan, Naira
Cc: Rex Johnson; mickie25@netzero.net
Subject: Re: [Histonet] Air purifiers
If you can taste DAB after working with it (either weighing of diluting it) it means that you need to wear an AIR MASK, the air purifier will not be enough. Remember that DAB is cancerigenous. René J.
--- On Mon, 9/8/08, Margaryan, Naira wrote:
From: Margaryan, Naira
Subject: [Histonet] Air purifiers
To: histonet@lists.utsouthwestern.edu
Cc: "mickie25@netzero.net" <'mickie25@netzero.net'>, "Rex Johnson"
Date: Monday, September 8, 2008, 11:39 AM
Hi histonetters,
Is there any Air purifiers for DAB?
I guess I am allergic to that reagent; I can test it after using.
I am OK to Formaldehyde, Xylene, Toluene, Glutaraldehyde, Alcohol, Acetone because I use them in the hood, but DAB is a step in autostainer.
Regards,
Naira
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