To make a 1-L 0.2 M phosphate buffer stock my recipe calls for 21.87 g Na2HPO4 (anhydrous dibasic) and 6.35 g NaH2PO4.H2O (monohydrate monobasic) dissolved in 1-L of water. (fairly standard procedure). I am sure my undergraduate chemistry professor is turning over in his grave, but I am embarrass to say that I am having difficulty figuring out how the amount of anhydrous dibasic and monohydrate monobasic was determined. Could someone please show me the calculations. I have played around with Henderson-Hasselbalch equation to no end.
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