I need the help of those with a talent in chemical calculation.
This formula is used in our institute since 1970, but nobody knows the
origin or a publication.
Formalin-formula: 8% buffered Formaldehyd
2 Liter 36% Formaldehyd
2 Liter Phosphatpuffer pH 7,8
6 Liter Aqua dest.
commercial Phosphatpuffer contents:
Natrium di-hydrogenphosphat monohydrat 1,810 g
di-Natrium monohydrogenphosphat dihydrat 3,756 g
Natriumchlorid 4,4 g
Water ad 1000
Phosphatebuffer has a molarity of 34,2 mmol/l.
Can anybody calculate the osmolarity of the ready formalin-solution for me?
What could be the advantages or disadvantages compared to Lillies 4% NBF or
Carson's 4% NBF?
Is the buffer-effect big enough in comparison to other formulas with 0,1 M
Pleas help, any input is appreciated.