[Histonet] What to do when there is no liquid nitrogen :p

From:"Heath, Nancy L."



Hi Everyone,
 
Does anyone have alternate methods for snap freezing muscle when there
is no liquid nitrogen?? I've been told to make a slurry with isopentane
& dry ice or to put isopentane in a plastic cup (?) and put into the
minus 70* freezer till it gets cold. Also how volatile / dangerous is
isopentane to work with??? Other than a flammable cabinet are there any
other things I need to know about isopentane???
 
Thanks,
Nancy
 
 
 
 
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