[Histonet] Re: paraformaldehyde grade

From:"David A. Wright"

Hi Mary, René, & Histonet

I agree with René completely about concentration & different
paraformaldehyde grades but you might want to consider a
couple of other issues too:
a) physical state.  A coarse prill is much easier to weigh out
without contamination than a fine powder.
b) filtration.  Before perfusing the brains you have to filter
out anything that might clog the capillaries. There's usually
a milky haze left after dissolving the paraformaldehyde which
you have to remove.  I imagine that a lower grade might have
more undissolved matter, and you have to change filters a lot
even with good stuff (approx every half liter).
David A. Wright PhD
University of Chicago Section of Neurosurgery

---- Original message ----
>Date: Thu, 11 Sep 2008 12:09:50 -0500 (CDT)
>From: histonet-request@lists.utsouthwestern.edu  
>Subject: Histonet Digest, Vol 58, Issue 13 Message: 1
>Date: Wed, 10 Sep 2008 10:10:42 -0700 (PDT)
>From: Rene J Buesa 
>Subject: Re: [Histonet] Paraformaldehyde
>To: histonet@lists.utsouthwestern.edu, "Mary P. Brownson"

>It does not really matter, BUT it will have to be taken into
consideration when calculating the final concentration you
want to use.
>René J.
>--- On Tue, 9/9/08, Mary P. Brownson  wrote:
>From: Mary P. Brownson 
>Subject: [Histonet] Paraformaldehyde
>To: histonet@lists.utsouthwestern.edu
>Date: Tuesday, September 9, 2008, 4:50 PM
>I plan to use paraformaldehyde for perfusion of rat brains. 
My question
>is; what grade paraformaldehde should I be using?  Reagent
grade, 95% or
>other?  Or does it not matter?

>Thank you,

>Mary Brownson
>School of Pharmacy
>University of Wyoming
>Laramie, WY 82070

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