I would beg to differ with Rene on this one. I don't believe antigen
retrieval is "by definition" using heat and sometimes pressure. I would
define antigen retrieval as any process that restores the original three
dimensional structure to a molecule altered by fixation (typically formalin
fixation) such that it can be recognized by the detecting antibody.
I have performed antigen retrieval by leaving slides overnight in the
refrigerator immersed in PBS + 0.05% Tween 20. I'm sure there are many other
ways to accomplish antigen retrieval, heat merely accelerates the process.
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Date: Fri, 5 Sep 2008 09:36:35 -0700 (PDT)
From: Rene J Buesa
Subject: Re: [Histonet] Antigen Retreival
To: Histonet@lists.utsouthwestern.edu, email@example.com,
Content-Type: text/plain; charset=iso-8859-1
You can always "open" the antigenic sites with enzymatic digestions. The
antigen retrieval is, by definition, using heat, and sometimes additional
pressure to have an even higher heat level.
--- On Fri, 9/5/08, firstname.lastname@example.org wrote:
Subject: [Histonet] Antigen Retreival
To: Histonet@lists.utsouthwestern.edu, email@example.com
Date: Friday, September 5, 2008, 11:06 AM
Does anyone out there have any methods of antigen retreival not using heat?
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