Does anyone have any experience with staining pig endothelium, specifically
keeping rings of blood vessels alive?
I am collecting fresh tissue from the slaughterhouse and incubating it with
tumour necrosis factor to induce VCAM-1 expression. However, I suspect that
the tissue quickly loses viability and this is why I canšt visualise any
VCAM with IHC.
Does anyone have any tips for keeping the rings of tissue
Alistair Lindsay MBChB MRCP
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