I fully agree with Isaac.
We all know that the liver always gives a background even if 4 steps of
blocking were used (H2O2, Avidin, Biotin, Protein Block).
Why in TMAs always we found a liver as a control sample, which gives us
Date: Sat, 15 Mar 2008 11:29:08 -0700
From: "Isaac Milos"
Subject: RE: [Histonet] Endogenous Peroxide Treatment -
To: "Marilyn Johnson" ,
Content-Type: text/plain; charset="us-ascii"
If you are running your IHC stains with an HRP detection kit with a
biotinylated secondary antibody, endogenous biotin within the tissue can
cause background staining to occur. Often times, you'll see endogenous
biotin in a tissue that is particularly bloody, such as liver, kidney,
You can use an avidin/biotin block before secondary antibody is applied
to account for this. You can find this block through many different
companies - mine (Cell Marque) offers a good version. The part number
is CMX222 and it can be ordered at 1-800-665-7284.
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