Brian,
I haven't seen your procedure for antigen retrieval before. I usually see 
heat/citrate buffer or some kind of protease treatment. It seems pretty 
straightforward. Have you ever used this procedure for keratins or 
basement membrane proteins?

Brian wrote: 
I have performed antigen retrieval by leaving slides overnight in the
refrigerator immersed in PBS + 0.05% Tween 20. I'm sure there are many 
other
ways to accomplish antigen retrieval, heat merely accelerates the process.


Thanks. 
Mel

Melville B. Vaughan, Ph. D.
Assistant Professor of Biology
Campus Coordinator, NSF Sure-Step program
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034
Telephone 405-974-5725
Fax 405-974-5726

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