[Histonet] Antigen retrieval 'tween night and next day


I haven't seen your procedure for antigen retrieval before. I usually see 
heat/citrate buffer or some kind of protease treatment. It seems pretty 
straightforward. Have you ever used this procedure for keratins or 
basement membrane proteins?

Brian wrote: 
I have performed antigen retrieval by leaving slides overnight in the
refrigerator immersed in PBS + 0.05% Tween 20. I'm sure there are many 
ways to accomplish antigen retrieval, heat merely accelerates the process.


Melville B. Vaughan, Ph. D.
Assistant Professor of Biology
Campus Coordinator, NSF Sure-Step program
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034
Telephone 405-974-5725
Fax 405-974-5726

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