[Histonet] AZF fixed bone marrows and IHC's

From:"Christine Jacobs"

Is anyone having problems with IHC staining after a combination os AZF fixation and decal? There was a recent string on the Histonet regarding B-Plus but I can't remember exactly what was discussed. We are experiencing problems with TdT staining. We get nothing on the core biopsies and only edge artifact staining on most clots. The FFPE thymus tissue looks GREAT. We are using Cell Marque's predilute antibody on the Ventana BenchMark XT. 
Any and all comments will be appreciated.
Chris Jacobs, HT(ASCP), QIHC
Jason Wheaton, HTL (ASCP)
NorDx Labs
Scarborough, ME

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