Re: [Histonet] paraffin processing problem

From:"Joe Nocito"

this is just a guess, but I would check the paraffin lines and/or the levels

----- Original Message ----- 
From: "Carla M Conway" 
Sent: Wednesday, September 19, 2007 10:54 AM
Subject: [Histonet] paraffin processing problem

> Hello,
> I have an LX120 tissue processor. The paraffin infiltration time is
> programmed for  three 1hour stations. Sometime last night a problem
> occurred during the paraffin cycle (unfortunately, we do not have a
> printout or record of when it happened).  This morning the Fill Failure
> alarm was sounding and tissues were not soaking in paraffin as they should
> have been. There was only  paraffin residue in the processing chamber, so 
> I
> know that the tissues were exposed to paraffin but don't know for how 
> long.
> What should I do? I do not want to overprocess them in paraffin, but don't
> want them underinfiltrated either. The tissues are staghorn sculpin hearts
> and livers.
> Thanks so much for your help,
> Carla Conway
> Western Fisheries Research Center
> 6505 NE 65th St.
> Seattle, WA 98115
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