Re: [Histonet] Re: Hydrogen Peroxide quenching

From:Rene J Buesa



You use it immediately after the HIER, before starting the IHC protocol.
  René J.

Daivik_Shah@rush.edu wrote:
  Hello friends,
I have question regarding Hydrogen peroxide quenching. Is this treatment 
applied in Immunohistostain after deparafinization or before application 
of secondary antibody? Is that affect any way to stain?

Thanks.
Daivik
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Daivik Shah, Histotechnologist, M.S. Biotechnology
RADC Laboratory, Cohn Building 436
Rush University Medical Center
Tel. 312-563-4891


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