Re: [Histonet] PFA & paraffin

From:Rene J Buesa

Try adding a few drops of liquid detergent to the water bath.
  René J.

Atoska Gentry  wrote:
  hello, does anyone have a tried & proven method for obtaining wrinkle 
free 6u cat brain sections that have been fixed in 4% PFA/0.1MPB , 
processed via standard for paraffin embedded sections? I briefly 
searched the histonet archive but after going thru approximately( 
x50)of 348 entries I figured there must be an easier/quicker way. Your 
prompt replies will be much appreciated. Atoska :-\

Atoska S. Gentry, B.S., HT(ASCP)
Research Assistant IV
Scott-Ritchey RSCH Center
College of Vet. Med
Auburn, AL 36849
PH (334) 844-5579
FAX (334) 844-5850

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