Re: [Histonet] Immuno's on Thin Prep specimens

From:Rene J Buesa

If using thin preps for IHC, you do NOT need to do HIER because they are acetone/air dried fixed, not formalin fixed. The rest of the IHC protocol is the same as for a FFPE tissue section. Your positive control refers to the epitope, so your regular positive control from a tissue section can be used, BUT you negative control has to be a thin prep from the same case simultaneously prepared.
  René J. wrote:
Is anyone doing IHC's on cytology specimens? If you are can you please
send staining and fixation protocol. Also, what do you use for your
positive and negative controls? Thank you

Andrea J Weiss BST CT (ASCP)
609 653 3577 Ext 4907
This transmittal from Shore Memorial Health System is for the sole use of
the intended recipient and may contain confidential and privileged
information. Any unauthorized review or use, including disclosure or
distribution is prohibited. If you are not the intended recipient, please
contact the sender and destroy all copies of the transmittal.

Histonet mailing list

Need a vacation? Get great deals to amazing places on Yahoo! Travel. 
Histonet mailing list

<< Previous Message | Next Message >>