Hi Patsy:
This week there was a discussion in Histonet about IHC on PAP smears and cytospins and using tissue sections as positive controls or not.
Roughly the positions were the following:
1- some accepted using FFPE tissue sections as (+) controls, after HIER
2- some suggested fixing the smears with NBF and cause the "crossslinkage" to do HIER afterwards
3- some advocated using ONLY known (+) smears or frozen sections as (+) controls, i.e. non-fixed tissues. The rational being a CAP instruction in which they direct to use controls treated the same as the case.
I think this is an important topic but I also think the "discussion" should be beyond an intelectual argument of each, because that will just expand what has been said this last week.
I think that with some time ahead some "hard evidence" ought to be presented for discussion, i.e. results obtained using the 3 "approaches" summarized above.
The panel then should view the slides, grade them and get to a determination. The slides should be reviewed/graded "blind", i.e. no one shoudl know the procedure s/he is evaluating.
Give consideration to this proposal.
René J.
patsy ruegg wrote:
This is a call for IHC questions to be discussed at the NSH S/C in Denver in
OCT.
If you have any burning questions you would like our panel of 9 to address
please email them to me ahead of time.
Thank you,
Patsy Ruegg
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #216
Aurora, CO 80010
720-859-4060
fax 720-859-4110
pruegg@ihctech.net
www.ihctech.net >
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #216
Aurora, CO 80010
720-859-4060
fax 720-859-4110
pruegg@ihctech.net
www.ihctech.net
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