Re: [Histonet] Hydrogen Peroxide Question

From:Patti Loykasek

We use H2O2 from the grocery store, works great. For FFPE, use it on depar.
slides for 5 min. However, I have found that on frozen tissue, some CD
markers are adversely affected by H2O2. On frozen tissue, we use an azide
block instead. I could send you the details or check histonet archives. Hope
this helps. 

Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA

> Hi Everyone,
> I wanted to post this for one of my co-workers.
> What percentage H2O2 is used to quench endogenous peroxidase in frozen
> section CD marker staining? how long?
> Thanks,
> Judi Ford
> Palo Alto, CA 
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