Peace be upon you,
I tried mouse monoclonal antibody against HIF-1 a  from Chemicon
International Inc. (Temecula, CA, USA, 1:100) and It was working nicely, I
would recommend you to use Tris-EDTA for retrival.
Good luck


-- 
Wael Mohamed Swelam
BDS (Egypt), Oral Pathology MSc (Egypt), Oral Pathology PhD (Japan),
Ass. Prof. Oral Biomedical Science dept. Division of Oral Pathology,
Faculty of Dentistry, King Faisal University, Dammam,
Saudi Arabia,
P.O: 1982/3441,
http://myprofile.cos.com/wmswelam
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