Re: [Histonet] Flash freezing tissue for transport to be cryostat sectioned at a later time

From:"Greg Dobbin"

Hi Carol,
I guess "quick" is no longer applicable here, but when you have time,
order the old fashion Peel-A-Way molds. Label each mold, place a little
OCT in the bottom and then add the tissue. Float thease molds then on a
foil boat in a safe receptical for liq. N2. As the bottom of the mold
starts to freeze, cover the tissue with more OCT and allow to freeze
completely. The tissue is now labelled, safe from freeze drying and
ready for mounting on a chuck (once the mold is removed of course) and
cutting. See full description in Biotechnic & Histochemistry 2003,
78(5): 279. "Notes and queries" section.

Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!

>>> "Wilson, Carol"  9/28/2007 3:28:01 PM >>>
Hi all, How would anyone out there suggest freezing various pieces of
cat tissue to be transported to another laboratory on dry ice for
cryostat sectioning when it gets to its destination?  We don't have a
cryostat or chucks on site, liquid nitrogen available.  We need to be
able to keep identity of each piece.  Suggestions quick please as the
necropsy team wants to stuff pieces of tissue into cryo vials and the
pieces are way to big to be stuffed and will certainly end up crushed.




Carol Wilson

Team Leader - Histology



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