Hi Carol,
I guess "quick" is no longer applicable here, but when you have time,
order the old fashion Peel-A-Way molds. Label each mold, place a little
OCT in the bottom and then add the tissue. Float thease molds then on a
foil boat in a safe receptical for liq. N2. As the bottom of the mold
starts to freeze, cover the tissue with more OCT and allow to freeze
completely. The tissue is now labelled, safe from freeze drying and
ready for mounting on a chuck (once the mold is removed of course) and
cutting. See full description in Biotechnic & Histochemistry 2003,
78(5): 279. "Notes and queries" section.
Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385
There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!
>>> "Wilson, Carol" 9/28/2007 3:28:01 PM >>>
Hi all, How would anyone out there suggest freezing various pieces of
cat tissue to be transported to another laboratory on dry ice for
cryostat sectioning when it gets to its destination? We don't have a
cryostat or chucks on site, liquid nitrogen available. We need to be
able to keep identity of each piece. Suggestions quick please as the
necropsy team wants to stuff pieces of tissue into cryo vials and the
pieces are way to big to be stuffed and will certainly end up crushed.
Thanks,
Carol
Carol Wilson
Team Leader - Histology
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Statement of Confidentiality
This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system.
D?claration de confidentialit?
Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
|