The AChE method in Filipe & Lake's book (Histochemistry in Pathology) is the one-step Karnovsky & Roots method, with inhibition of pseudocholinesterase by iso-OMPA and an intensification step using osmium tetroxide. It's intended for diagnosis of Hirschprung's disease - minimally fixed cryosections of rectal biopsies.
The Karnovsky-Roots method is very reliable and in my experience (with brain and muscle) preferable to the Koelle-type methods. If you don't like using osmium tetroxide, there's an alternative intensification procedure using Nickel-DAB. See Tago G et al 1986 J. Histochem. Cytochem. 34: 1431-1438. The ChE inhibitor iso-OMPA is pretty toxic=2E Depending on the nature of your investigation, you may not need it=2E
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
Date: Friday, September 21, 2007 7:31
Subject: [Histonet] Acetylcholinesterase protocol and fixation
> Another question...
> Looking for Acetylcholinesterase protocols I´ve found that probably
> Filipe and Lake´s is the most used, is this information correct?
> And... found using Karnovsky fixation or formaldehyde/calcium
> acetate.. which one is the best??
> Tks again
> Ms. Kátia Catunda
> +55 12 3203-0612 (direto)
> +55 12 3203-0633 (PABX)
> Histonet mailing list
Histonet mailing list