Hi Valeria,
I have tried 2 of Santa Cruz's NOTCH-1 abs with no success on FFPE
sections (#s 6014 and 9170) but they don't claim to work for this
application or not with my system, i.e. raised in goat. The one my
researcher DIDN'T buy is the 6014-R (raised in rabbit) that might have
actually worked with my system. At my researcher's insistence, I did a
1:5 dilution, 10" HIER in a pressure cooker and amplification of the
primary ab. All I got was nicely delineated epithelial cells against a
nasty brown background but no differentiation between normal and
malignant prostate cells, which was the focus of his research.
Good luck!.
Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Valeria
Berno
Sent: Tuesday, September 18, 2007 10:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] notch-1
Hello All,
I was wondering if there is anyone out there who has successfully done
Notch-1 staining on paraffin sections. If there is, would they be able
to
could you tell me of the antibody, fixation/embedding protocol, and
subsequent
treatments, if any. I have tried 3 Santa cruz antibodies unsuccessfully
and
we were wondering if it is something about the antibodies or the
protocol!!!
Thanks a lot
Valeria
Berno Valeria, PhD
EMBL Monterotondo Outstation
via Ramarini 32
00015 Monterotondo Scalo (RM)
Italy
Tel: +39 06 90091 287
Fax: +39 06 90091 272
HYPERLINK "mailto:valeria.berno@embl.it"valeria.berno@embl.it
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