RE: [Histonet] negative controls

From:"Simmons, Christopher"

CAP requires a negative control for each test.
We utilize a micro-array sausage control that allows our slide count to
be reduced as the 16-20 tissue types on each of our 3 control slides
validated the 350 immunos we have in our battery.
Remember to order a negative control not just with each block or part
type, but with additional reflex orders as well.

Chris Simmons
Lead Technologist
UPMC Presbyterian Histology
412.647.7660 desk
13242 pager

-----Original Message-----
[] On Behalf Of Horn,
Hazel V
Sent: Monday, September 17, 2007 8:45 AM
Subject: RE: [Histonet] negative controls

We do a negative control on each block and sometimes we do 2.   If
several antibodies are ordered on one block and their species are
different, (i.e. rabbit, mouse) we run one negative mouse and one
negative rabbit.

Each block should have its own negative control.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3912

visit us on the web at:

-----Original Message-----
[] On Behalf Of
Sent: Friday, September 14, 2007 2:36 PM
Subject: [Histonet] negative controls

How many non-CAP labs out there are doing negative controls on every
block that you do an antibody on?

If you have a case that goes A-J and they are all 1 block each and you
do an AE1/AE3 on blocks B,C,F,& J, will you do a negative on every block
or just one for the entire case?

This has been an ongoing debate with us.? We typincally only do 1
negative control for the case and given the above example, it might be
on block A.? We ususally cut an extra on every block we cut and when the
IHC is requested we just go and pull it and for the negative, we just
grab whatever slide is left to run the negative on.? What is everyone
else doing?? 

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