RE: [Histonet] Re: Hydrogen Peroxide quenching

From:"Edwards, R.E."

It is   not  a  dumb  question at  all, every lab does  things  their  way for  a  variety of  reasons, the  main  one  being of course "because  we  have  always  done  it  this  way, and  it  works for  us", with  the  real  reasons  why  things are done  in  a  particular  way are lost in the  mists  of  times long long  ago, when the  world  was  young and  global warming had  not been  thought  about. 

-----Original Message-----
From: [] On Behalf Of Ford, Judi
Sent: 26 September 2007 23:04
To: Rene J Buesa;;
Subject: RE: [Histonet] Re: Hydrogen Peroxide quenching

Okay, so I'm confused. Some labs do the hydrogen peroxide step before HIER and others afterwards, why? Also, are there differences using hydrogen peroxide diluted in water/methanol/or PBS? Some bring paraffin slides down to absolute alcohol then do the hydrogen peroxide step where others wait until bringing the slides to water before doing the step. Is there some rule of thumb, or do you just figure out which procedure works for that specific antibody in your lab?

I'm still pretty new to this, so sorry if this seems like a dumb question.

Judi F.
Palo Alto, CA

-----Original Message-----
From: [] On Behalf Of Rene J Buesa
Sent: Wednesday, September 26, 2007 2:49 PM
Subject: Re: [Histonet] Re: Hydrogen Peroxide quenching

You use it immediately after the HIER, before starting the IHC protocol.
  René J. wrote:
  Hello friends,
I have question regarding Hydrogen peroxide quenching. Is this treatment applied in Immunohistostain after deparafinization or before application of secondary antibody? Is that affect any way to stain?

Daivik Shah, Histotechnologist, M.S. Biotechnology RADC Laboratory, Cohn Building 436 Rush University Medical Center Tel. 312-563-4891

This message and any attachments contain information for the exclusive use of the individual or entity to whom it is addressed and may contain information that is privileged, confidential and/or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, or the employee or agent, you are hereby notified that any distribution or copying of this communication is strictly prohibited. If you received this message in error, please phone me immediately at 312-563-4891.
Histonet mailing list

Got a little couch potato? 
Check out fun summer activities for kids.
Histonet mailing list

Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>