[Histonet] whole mount

From:"Valeria Berno"




Hi,
I was just wondering if it is common to use Sudan Black or other
autofluorescence quenching method on whole mounting.It is the first time I
am approaching whole mount immuno!!.

Is there any special suggestion as my antigene has a very low expression??

thanks in advance

Valeria 
 
Berno Valeria, PhD
 

EMBL Monterotondo Outstation

via Ramarini 32

00015 Monterotondo Scalo (RM)

Italy

Tel:  +39 06 90091 287

Fax: +39 06 90091 272

HYPERLINK "mailto:valeria.berno@embl.it"valeria.berno@embl.it

 

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