[Histonet] mucin stain on matrigel-coated cultured cells

From:Kim Merriam



Hello,Does anyone have a recommendation for a mucin stain for cultured cells on matrigel-coated slides?  An investigator at my site is interested in this.  I am worried that 1) the Schiff reagent will be too harsh for the cells and 2) that the matrigel itself might be PAS-positive.  I think IHC or IF might be the way to go, instead of a special stain.Any thoughts?Kim Kim Merriam, MA, HT(ASCP)Cambridge, MA       =0A____________________________________________________________________________________Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games.http://sims.yahoo.com/  
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