Dear All,
I am doing DIG-labeled RNA in situ hybridizations and noticed that some
protocols use MABT buffer (0.1M maleic acid, 0.15M NaCl, 0.1% Tween-20,
pH 7.5) to dilute AP-conjugated anti-DIG antibody, while the others use
TNT buffer (0.1M Tris, 0.15M NaCl, 0.1% Tween-20, pH7.5). The only
difference between these two buffers is Maleic acid vs. Tris.
I used Tris-based buffer for my Ab solution and it worked for me. I am
just curious about the difference between maleic acid and Tris. Does
anyone know if there is any advantage of choosing maleic acid over Tris?
Thanks for your input.
CK.
==================================
Chengkang Zhang Ph.D.
Room 357, MedSurge II
19182 Jamboree Rd,
Department of Pharmacology
University of California, Irvine
Irvine, CA 92697-4625
Email: chengkaz@uci.edu
Tel: (949)-824-1902 (lab)
Fax: (949)-824-4855
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