I try to use a different primary antibody other than mouse and make sure
that the secondary is not mouse either.  I also use a universal protein
block and a rodent block reagent from Biocare that has shown some promise in
my lab.  I do a lot of work with mouse xenograft tissues so I get high
background with most staining.  These reagent changes for us have made at
least a noticeable difference.


Aprill Watanabe, B.S.
Research Associate
Tissue Microarray Center (TMA)
Translational Genomics Research Institute (TGen)
602-343-8822
awatanabe@tgen.org
www.tgen.org


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