I try to use a different primary antibody other than mouse and make sure
that the secondary is not mouse either. I also use a universal protein
block and a rodent block reagent from Biocare that has shown some promise in
my lab. I do a lot of work with mouse xenograft tissues so I get high
background with most staining. These reagent changes for us have made at
least a noticeable difference.
Aprill Watanabe, B.S.
Tissue Microarray Center (TMA)
Translational Genomics Research Institute (TGen)
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