I got the recommendation to fix the slides for 10-20 min in NBF and run them
then in the same way as the paraffin slides. The pro is, that you don't have
to modify your usual protocols. Also it has been said, that some of the
antibodies bring better results with HIER, even if there was no
formalinfixation first.
But my personal exerperience is very small. 

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754

-----Ursprüngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von
AWeiss@shorememorial.org
Gesendet: Freitag, 28. September 2007 13:24
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Immuno's on Thin Prep specimens


Is anyone doing IHC's on cytology specimens?  If you are can you please
send staining and fixation protocol.   Also, what do you use for your
positive and negative controls?  Thank you

Andrea J Weiss BST CT (ASCP)
Cytotechnologist
609 653 3577 Ext 4907
aweiss@shorememorial.org
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