Re: [Histonet] toluidine staining faded!

From:Geoff McAuliffe

I suggest that you dehydrate with 2 changes of acetone, then clear in 
fresh xylene and coverslip.


Moran Elishmereni wrote:
> Hello histonetters,
> I stained some murine skin sections for toluidine blue, and mast cells were
> very easy to detect, however, after a few days the staining seemed to have
> faded. Following the tol blue staining, I briefly put the sections in
> alcohol (95%, 100%, 100%), then in xylene substitute, then air dryed and
> mounted with mounting media. What did I do wrong? I am very new
> at histology, so forgive my simple question...
> Thanks,
> Moran

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583

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