Re: [Histonet] toluidine staining faded!

From:Rene J Buesa

  My first concern is: if you dehydrated and placed in xylene substitute, WHY did you air dried them? After you place them in xylene substitute you can coverslip them UNLESS your mounting medium is incompatible wit the xylene substitute, in which case you should not use that substitute or change to another mounting medium.
  Toluidine blue fading can be cause by low pH and the cause could be the mounting medium that is not neutral (pH 7). 
  René J.

Moran Elishmereni  wrote:
  Hello histonetters,

I stained some murine skin sections for toluidine blue, and mast cells were
very easy to detect, however, after a few days the staining seemed to have
faded. Following the tol blue staining, I briefly put the sections in
alcohol (95%, 100%, 100%), then in xylene substitute, then air dryed and
mounted with mounting media. What did I do wrong? I am very new
at histology, so forgive my simple question...


Moran Elishmereni

Department of Pharmacology and Experimental Therapeutics
School of Pharmacy, Faculty of Medicine
The Hebrew University of Jerusalem
POB 12065
Jerusalem 91120, ISRAEL
Tel: 972-2-675-8746
Fax: 972-2-675-8144
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