Re: [Histonet] IHC anti-cleaved caspase 3

From:Cheryl Cross

I will second the Cell Signaling antibody - we have tried antibodies  
from Promega (which gave tons of background staining)...then via this  
site i was pointed to Cell Signaling's monoclonal which we tried; the  
polyclonal actually has been working very well with minimal  
background (we were getting lots of respiratory epithelium and  
endothelium lighting up in control animals). I have been using it in  
mice with this protocol:

primary antibody 60 minutes, dilution 1:125
Rabbit envision
DAB plus

Hope this helps!

Cheryl Cross, DVM, Dipl. ACVP
University Corporation for Atmospheric Research
College of Veterinary Medicine
University of Tennessee Department of Pathology
2407 River Drive, Room A201
Knoxville, TN 37996-4542
(423) 967-2724
fax: 865-974-5616


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