In a past life, I used to use Alcian Blue for GAG staining (I can't remember if it was pH 1 or pH 2.5); we used to fix the tissues in Methacarn and that made a big difference in GAG retention (vs NBF).Good luck,Kim=0A Kim Merriam, MA, HT(ASCP)Cambridge, MA----- Original Message ----From: "AGrobe2555@aol.com" To: histonet@lists.utsouthwestern.eduSent: Tuesday, September 4, 2007 4:32:30 PM=0ASubject: [Histonet] Alcain blue vs. Movats for GAG stainingHello All,Does anyone have a handle how GAG staining by Movats Pentachrome compares  to low pH Alcian blue staining?  I was asked to stain for GAGs in  vascular sections (blood vessels and grafts), and was wondering which would be  the best method (please suggest other methods).  I suspect there won't  be much staining for GAGs anyway.  Please correct me if this is  incorrect, as I have little experience in this area.Thanks,Albert ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet       ____________________________________________________________________________________Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase.http://farechase.yahoo.com/
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