I cut my tissue and let it air dry for 30 minutes, then acetone fix for 30
minutes at room temperature, then 3 changes of PBS for 5 minutes each before
applying the antibody and I rarely have problems.
----- Original Message -----
From: "Mauricio Avigdor"
Sent: Thursday, September 06, 2007 2:41 PM
Subject: [Histonet] Acetone fixation for immunofluorescence protocols
> I am having a bit of difficulty with an immunofluorescence protocol (IF).
> am using fresh tissue fixed for 10 minutes in cold acetone. Are there any
> fixatives that are well suited to IF protocols? Has anyone attempted to
> stain unfixed tissue?
> Histonet mailing list
Histonet mailing list