Mauricio,
I cut my tissue and let it air dry for 30 minutes, then acetone fix for 30 
minutes at room temperature, then 3 changes of PBS for 5 minutes each before 
applying the antibody and I rarely have problems.

JTT
----- Original Message ----- 
From: "Mauricio Avigdor" 
To: 
Sent: Thursday, September 06, 2007 2:41 PM
Subject: [Histonet] Acetone fixation for immunofluorescence protocols


> Greetings:
>
> I am having a bit of difficulty with an immunofluorescence protocol (IF). 
> I
> am using fresh tissue fixed for 10 minutes in cold acetone. Are there any
> fixatives that are well suited to IF protocols? Has anyone attempted to
> stain unfixed tissue?
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