RE: [Histonet] protocol for clearing tissue!

From:"Rittman, Barry R"

Oil of wintergreen has been used extensively in the past for clearing
even large blocks of tissue containing blood vessels visualized with
India ink, latex etc. So this would probably work.

-----Original Message-----
[] On Behalf Of Vest,
Sent: Thursday, September 06, 2007 12:51 PM
Subject: [Histonet] protocol for clearing tissue!

Dear histonet:

One of the pathology residents in our department has asked me to post
this question.


I am in search of a protocol for clearing tissue. This technique is used
commonly in vascular research to visualize blood vessels. I want to use
it to clear mouse lung tissue that contains blue-stained
(B-galactosidase activity) tumor nodules. Any suggestions?



Luther Vest (HT ASCP)

VA-MD Regional College of Veterinary Medicine

Duckpond Dr. Phase III

Blacksburg, Va 24061-0443



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