I have had a Nemesis for almost 2 years now and would be happy to answer any questions. I run a histology core facility for a group of brain tumor researchers. I stain human only, animal only and human implanted into animal sections. I use the Nemesis in the open mode only, no bar coding. I use primarily Biocare reagents, but few Biocare antibodies. I have done multiple stains on the instrument, including triple and quadruple. I have no complaints about the instrument and find it versatile in every way, which is what I need. All aspects of support are excellent, and I think that Biocare reagents, especially the newer Promark reagents are life savers to people staining xenografts, like myself.
I have had past extensive experience with the Biogenex i6000 and the Dako Immunostainer, so I have decent frames of reference.
I would be happy to respond to any questions.
Hermelin Brain Tumor Center
Henry Ford Hospital
From: "Sebree Linda A." LSebree@uwhealth.org
Date: Thu, 13 Sep 2007 09:11:39 -0400
To: "Christine Tambasco" email@example.com
Subject: RE: [Histonet]immuno stainers
> Please post replies to Histonet.
> Linda Sebree, HT(ASCP)
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> 600 Highland Ave.
> Madison, WI 53792
> FAX: (608)262-7174
> -----Original Message-----
> From: firstname.lastname@example.org > [mailto:email@example.com] On Behalf Of > Christine Tambasco
> Sent: Wednesday, September 12, 2007 4:26 PM
> To: firstname.lastname@example.org; email@example.com
> Subject: Re: [Histonet]immuno stainers
> Anybody have an experiences with the Nemisis from BioCare? > Would
> appreciate any feed back. Thanks
> Christine Tambasco, HT (ASCP)
> St Marys Hospital, Amsterdam NY
> From: "Mike Schlicht"
> Subject: Re: [Histonet] Special Stainers
> Date: Wed, 12 Sep 2007 12:51:34 -0700
> >We are currently using the DAKO Artisan for silver stains, and
> >some problems with uneven staining every now and then, usually
> >the dispensers are close to being empty, so we stop using the kit
> >when there are 5 tests remaining to help reduce some of the
> >problems. We had a demo of the Nexus stainer, and found that the
> >result were about the same.
> >San Luis Obispo, CA
> >>Message: 15
> >>Date: Tue, 11 Sep 2007 18:32:37 -0500
> >>From: "Rebecca Johnson"
> >>Subject: [Histonet] Special Stainers
> >>To: "histonet"
> >>Message-ID: <011a01c7f4cc$09fefcc0$7b48f9d8@CHURCH>
> >>Content-Type: text/plain; charset="iso-8859-1"
> >>What special stainers is everyone using? I now have a Ventana
> >>Nexus. Bought in 1999. I know this is old, but it has been
> >>maintained well. We have had problems with it several times over
> >>the years. Mostly with sliver stains or uneven staining and no
> >>staining. I would like to know what others are using and your
> >>experiences are. Thanks for all you help.
> >Histonet mailing list
> Kick back and relax with hot games and cool activities at > the
> Messenger Café.
> 1. http://g.msn.com/8HMBENUS/2740??PS=47575
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