RE: [Histonet]immuno stainers

From:"Nancy Lemke"

Hi All,
I have had a Nemesis for almost 2 years now and would be happy to answer any questions.  I run a histology core facility for a group of brain tumor researchers.  I stain  human only, animal only and human implanted into animal sections.  I use the Nemesis in the open mode only, no bar coding.  I use primarily Biocare reagents, but few Biocare antibodies.  I have done multiple stains on the instrument, including triple and quadruple.  I have no complaints about the instrument and find it versatile in every way, which is what I need.  All aspects of support are excellent, and I think that Biocare reagents, especially the newer Promark reagents are life savers to people staining xenografts, like myself.
I have had past extensive experience with the Biogenex i6000 and the Dako Immunostainer, so I have decent frames of reference.
I would be happy to respond to any questions.

Nancy Lemke
Research Coordinator
Hermelin Brain Tumor Center
Henry Ford Hospital
-----Original message-----
From: "Sebree Linda A."
Date: Thu, 13 Sep 2007 09:11:39 -0400
To: "Christine Tambasco"
Subject: RE: [Histonet]immuno stainers

> Please post replies to Histonet.
> Linda Sebree, HT(ASCP)
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> A4/204-3224
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> FAX: (608)262-7174
> -----Original Message-----
> From: > [] On Behalf Of > Christine Tambasco
> Sent: Wednesday, September 12, 2007 4:26 PM
> To:;
> Subject: Re: [Histonet]immuno stainers
>    Anybody  have  an  experiences  with  the  Nemisis from BioCare? > Would
>    appreciate any feed back. Thanks
>    Christine Tambasco, HT (ASCP)
>    St Marys Hospital, Amsterdam NY
>        ______________________________________________________________
>      From:  "Mike Schlicht" 
>      To:  
>      Subject:  Re: [Histonet] Special Stainers
>      Date:  Wed, 12 Sep 2007 12:51:34 -0700
>      >We  are  currently  using  the DAKO Artisan for silver stains, and
>      have
>      >some  problems  with  uneven  staining every now and then, usually
>      when
>      >the dispensers are close to being empty, so we stop using the kit
>      >when there are 5 tests remaining to help reduce some of the
>      >problems.  We had a demo of the Nexus stainer, and found that the
>      >result were about the same.
>      >Mike
>      >San Luis Obispo, CA
>      >
>      >
>      >>Message: 15
>      >>Date: Tue, 11 Sep 2007 18:32:37 -0500
>      >>From: "Rebecca Johnson" 
>      >>Subject: [Histonet] Special Stainers
>      >>To: "histonet" 
>      >>Message-ID: <011a01c7f4cc$09fefcc0$7b48f9d8@CHURCH>
>      >>Content-Type: text/plain; charset="iso-8859-1"
>      >>
>      >>What special stainers is everyone using?  I now have a Ventana
>      >>Nexus. Bought in 1999.  I know this is old, but it has been
>      >>maintained well. We have had problems with it several times over
>      >>the years. Mostly with sliver stains or uneven staining and no
>      >>staining.  I would like to know what others are using and your
>      >>experiences are. Thanks for all you help.
>      >>raj
>      >>
>      >
>      >
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>    [1]Kick  back  and  relax  with  hot  games and cool activities at > the
>    Messenger Café.
> References
>    1.
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